Determination of a Favorable Medium for Detection of Fungal Extracellular Protease.

• PARK JONG-CHUL

  Department of Medical Engineering, College of Medicine, Yonsei University

• HAN DONG-WOOK

  Department of Medical Engineering, College of Medicine, Yonsei University

• HWANG YU-SHIK

  Department of Medical Engineering, College of Medicine, Yonsei University

• LEE HUN-JUN

  Hygiene and Microbiological Test and Research Center

• TAKATORI KOSUKE

  National Institute of Health Sciences

• SUH HWAL

  Department of Medical Engineering, College of Medicine, Yonsei University

抄録

To determine a favorable medium for detecting fungal extracellular protease, a plate method was examined. In this study, 12 fungal strains were tested with 4 different types of Czapek Dox agar medium containing skim milk, and the clear zones around the colonies were compared on the different types of medium. Among them, 9 strains produced extracellular proteases. Triton X-100 was required to detect an extracellular protease fromAlternaria alternata, while in case of Peniciliium frequentans, saccharose was required. It was found that the media supplemented with both Triton X-100 and saccharose gave acceptable results in terms of extracellular protease detection.

収録刊行物

• Ｂｉｏｃｏｎｔｒｏｌ　Ｓｃｉｅｎｃｅ

  Ｂｉｏｃｏｎｔｒｏｌ　Ｓｃｉｅｎｃｅ 4 (2), 91-95, 1999

  日本防菌防黴学会

キーワード

• Extracellular protease
• Plate method
• Triton X-100
• Saccharose

詳細情報

• CRID: 1390282679440511104
• NII論文ID: 10011317522
• NII書誌ID: AA11169621
• DOI: 10.4265/bio.4.91
• ISSN: 18840205
• ISSN: 13424815
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可