Simultaneous Determination of Choline and Acetylcholine Based on a Trienzyme Chemiluminometric Biosensor in a Single Line Flow Injection System

• KIBA Nobutoshi

  Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

• ITO Seiji

  Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

• TACHIBANA Masaki

  Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

• TANI Kazue

  Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

• KOIZUMI Hitoshi

  Department of Applied Chemistry and Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

抄録

A detector for the simultaneous determination of choline (Ch) and acetylcholine (ACh) based on a sensitive trienzyme chemiluminometric biosensor in a single line flow injection (FI) system is described. Immobilized choline oxidase (ChOx), immobilized peroxidase (POx), immobilized acetylcholinesterase, and coimmobilized ChOx/POx were packed, in turn, in a transparent ETFE tube (1 mm i.d., 75 cm) and the tube was placed in front of a photomultipier tube as a flow cell. Two-peak response was obtained by one injection of the sample solution. The first and second peaks were dependent on the concentrations of Ch and ACh, respectively. The influence of some experimental parameters such as flow rate, amounts of immobilized enzymes on the behavior of the sensor was studied in order to optimize the sensitivity, sample throughput and resolution. Calibration curves were linear at 1 - 1000 nM for Ch and 3 - 3000 nM for ACh. The sample throughput was 25/h without carryover. The FI system was applied to the simultaneous determination of Ch and ACh in rabbit brain tissue homogenates.

収録刊行物

• Analytical Sciences

  Analytical Sciences 19 (12), 1647-1651, 2003

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390282679234029056
• NII論文ID: 130004440669
• NII論文ID: 10011874273
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.19.1647
• COI: 1:CAS:528:DC%2BD3sXpvV2nt74%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 6774785
• PubMed: 14696930
• Web Site: http://id.ndl.go.jp/bib/6774785
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6774785
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.19.1647.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.19.1647/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/19/12/19_12_1647/_pdf
• 本文言語コード: en
• データソース種別:
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