Control of Adhesion and Removal of Bacterial Cells from Stable Suspension Using Surface Characteristics

• HAYASHI Hiroshi

  Department of Chemical Engineering, Waseda University

• NIHEI Tomoya

  Department of Environment and Resources Engineering, Waseda University

• SEIKI Hiroshi

  Department of Environment and Resources Engineering, Waseda University

• TSUNEDA Satoshi

  Department of Chemical Engineering, Waseda University

• HIRATA Akira

  Department of Chemical Engineering, Waseda University

• SASAKI Hiroshi

  Department of Environment and Resources Engineering, Waseda University

Bibliographic Information

Other Title

• 界面特性を利用した細菌細胞の付着・脱着制御法
• カイメン トクセイ オ リヨウ シタ サイキン サイボウ ノ フチャク ダッチャク セイギョホウ

Abstract

Rapid collection and removal of gram-negative heterotrophic bacterial strain, Escherichia coli, was investigated by utilizing the interfacial interaction between cell and solid substratum. Both E.coli collection from stable suspension and its successive removal were performed by a column bed packed with fibrous ferro-nickel slag, which was employed as the collector media of bacterial cells. In the cell collection tests, little cell recovery was obtained at neutral pH condition where both E.coli and FS had negative surface potential. On the other hand, E.coli collection gradually increased as the pH decreased. These findings were explained by the suppression of electrostatic repulsive interaction between E.coli and FS led to an improvement in cell attachment to FS surface. Cell collection capacity Γ_max exponentially increased as the interfacial interaction energy minimum V_min became lower, indicating that surface characteristics played crucial roles in cell attachment mechanism. Moreover, part of the E.coli cells that had adhered to FS surface were effectively removed when the eluting solutions were alkaline conditions; hence reversible cell detachment from FS was possible by controlling the electro-repulsive force. Cyclic E.coli collection/removal tests demonstrated that cell collection and successive removal were repeatedly carried out al least six times, although about 40 mg of E.coli cell made a firm and irreversible attachment on FS. E.coli collection/removal behaviors in the present experiments were generally in good agreement with electrokinetic properties of cell and FS, suggesting that cell recovery based on surface characteristics is a promising method, especially for stable bacterial suspension.

Journal

• Shigen-to-Sozai

  Shigen-to-Sozai 120 (1), 54-59, 2004

  The Mining and Materials Processing Institute of Japan

Keywords

• Surface Characteristics
• <i>E.coli</i>
• Electrophoretic Mobility
• Fibrous Slag
• Attachment
• Detachment

Details

• CRID: 1390282681026751616
• NII Article ID: 10011983412
• NII Book ID: AN10062646
• DOI: 10.2473/shigentosozai.120.54
• COI: 1:CAS:528:DC%2BD2cXhtFaisLw%3D
• ISSN: 18806244
• ISSN: 09161740
• NDL BIB ID: 6834654
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6834654
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed