Co-localization of Rab4 with endocytosis-related proteins in the rat parotid glands.
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- Nashida Tomoko
- Department of Biochemistry, The Nippon Dental University School of Dentistry at Niigata
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- Yoshie Sumio
- Department of Histology, The Nippon Dental University School of Dentistry at Niigata
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- Imai Akane
- Department of Biochemistry, The Nippon Dental University School of Dentistry at Niigata
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- Shimomura Hiromi
- Department of Biochemistry, The Nippon Dental University School of Dentistry at Niigata Advanced Research Center, The Nippon Dental University School of Dentistry at Niigata
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Small GTP-binding proteins have been implicated in the regulation of vesicular traffic. We investigated the localization of Rab4 in the rat parotid glands by Western blotting and light-microscopic immunohistochemistry. Rab4 was localized mainly on the intracellular membranes in the subapical-actin terminal web, but was not present in the basolateral region both in acinar and ductal cells. Actin, α-adaptin, Rab5A and aquaporin5 were detected in the Rab4-containing intracellular membrane fraction prepared using anti-Rab4 antibody covalently coupled to magnetic beads. Detection of actin indicated that the Rab4-containing intracellular membranes were attached to the actin filaments. Although α-adaptin was immunohistochemically distributed along the plasma membrane, this protein coexisted with Rab4 only at the apical region. Rab5A immunoreactivity was distributed all around the cytoplasm. These findings suggested that Rab4 participates in endocytosis at the apical membrane of parotid glands.
収録刊行物
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- Archives of Histology and Cytology
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Archives of Histology and Cytology 66 (1), 45-52, 2003
国際組織細胞学会
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詳細情報 詳細情報について
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- CRID
- 1390001206407455104
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- NII論文ID
- 10011993082
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- NII書誌ID
- AA1068990X
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- COI
- 1:CAS:528:DC%2BD3sXjt1ygsbw%3D
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- ISSN
- 13491717
- 09149465
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- PubMed
- 12703553
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- 使用不可