ATP-induced [Ca2+]i changes in the human corneal epithelium.
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- Kubo-Watanabe Shoko
- Department of Histology, Iwate Medical University School of Medicine Departments of Ophthalmology, Iwate Medical University School of Medicine
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- Goto Shinichi
- Department of Histology, Iwate Medical University School of Medicine
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- Saino Tomoyuki
- Department of Histology, Iwate Medical University School of Medicine
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- Tazawa Yutaka
- Departments of Ophthalmology, Iwate Medical University School of Medicine
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- Satoh Yoh-ich
- Department of Histology, Iwate Medical University School of Medicine
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Abstract
ATP, when leaked from damaged cells, is capable of eliciting responses in neighboring cells. A better understanding of the mechanism of this response is essential for designing therapeutic strategies for disease, there have been only a limited number of studies on the effect of ATP on the human cornea. We examined ATP-induced intracellular Ca2+ ([Ca2+]i) changes in the human corneal epithelia, cultured to near confluence. Cells were loaded with the Ca2+ indicators, Indo-1 or Fluo-4, and [Ca2+]i was monitored. ATP was found to induce an increase in [Ca2+]i, which was initiated from the perinuclear region and the nuclear envelope per se, and then propagated gradually towards the periphery. Intranuclear Ca2+ was momentarily increased. UTP elicited an identical response, but adenosine and α, β-methylene ATP had no effect. Pretreatment with U73122 or thapsigargin inhibited the ATP-induced increase in [Ca2+]i. When a cell was topically stimulated with ATP, the [Ca2+]i increase spread beyond the cell boundary. The intercellular communications that accompanied the [Ca2+]i changes were inhibited by octanol. We conclude that extracellular ATP in the human cornea caused the mobilization of Ca2+ from intracellular Ca2+ stores (e. g. the endoplasmic reticulum and nuclear envelope) via P2Y purinoceptors of the epithelial cell. The response to ATP appears to spread to neighboring regions through gap junctions in the epithelium.
Journal
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- Archives of Histology and Cytology
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Archives of Histology and Cytology 66 (1), 63-72, 2003
International Society of Histology and Cytology
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Keywords
Details 詳細情報について
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- CRID
- 1390001206410813696
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- NII Article ID
- 10011993132
- 30012644160
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- NII Book ID
- AA1068990X
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- COI
- 1:CAS:528:DC%2BD3sXjt1ygsbo%3D
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- ISSN
- 13491717
- 09149465
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- PubMed
- 12703555
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed