Transcriptional, Functional and Cytochemical Analyses of the veg Gene in Bacillus subtilis

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  • Fukushima Tatsuya
    Department of Applied Biology Faculty of Textile Science and Technology, Shinshu University
  • Ishikawa Shu
    Department of Applied Biology Faculty of Textile Science and Technology, Shinshu University
  • Yamamoto Hiroki
    Department of Applied Biology Faculty of Textile Science and Technology, Shinshu University
  • Ogasawara Naotake
    Graduate School of Information Science, Nara Institute of Science and Technology
  • Sekiguchi Junichi
    Department of Applied Biology Faculty of Textile Science and Technology, Shinshu University

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  • Transcriptional, Functional and Cytochemical Analyses of the <i>veg</i> Gene in <i>Bacillus subtilis</i>

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A Bacillus subtilis veg mutant exhibited a small reduction of absorbance, a large reduction of hexosamine release, and slow dipicolinic acid release from spores during germination with L-alanine as a germinant. But veg spores exhibited normal resistance to chloroform, 2-propanol, lysozyme, and heat. SDS-polyacrylamide gel electrophoresis of spore coat proteins revealed no difference in coat proteins between the wild type and the veg mutant. Northern and veg-lacZ fusion analyses indicated that the veg gene is transcribed in both the vegetative growth and sporulation phases, and primer extension analysis indicated an identical transcriptional start point in both phases. The upstream sequence suggests that veg is transcribed by EσA RNA polymerase. Veg-GFP fusion protein was detected for vegetative cells and spores, but the fluorescence of mother cells disappeared completely in the late sporulation phase. Decoated spores containing Veg-GFP exhibited a strong green fluorescence in the core, but much weaker fluorescence in the cortex.

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