Germ Cell-Specific Expression of Microphthalmia-Associated Transcription Factor mRNA in Mouse Testis
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- Saito Hideo
- Department of Molecular Biology and Applied Physiology Department of Urology, Tohoku University School of Medicine
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- Takeda Kazuhisa
- Department of Molecular Biology and Applied Physiology
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- Yasumoto Ken-ichi
- Department of Molecular Biology and Applied Physiology
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- Ohtani Haruo
- Department of Pathology, Tohoku University School of Medicine Research Division and Clinical Laboratory, Mito National Hospital
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- Watanabe Ken-ichi
- Department of Molecular Biology and Applied Physiology
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- Takahashi Kazuhiro
- Department of Molecular Biology and Applied Physiology
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- Fukuzaki Atsushi
- Department of Urology, Tohoku University School of Medicine
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- Arai Yoichi
- Department of Urology, Tohoku University School of Medicine
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- Yamamoto Hiroaki
- Biological Institute, Graduate School of Life Sciences, Tohoku University
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- Shibahara Shigeki
- Department of Molecular Biology and Applied Physiology
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抄録
The gene coding for microphthalmia-associated transcription factor (Mitt) contains many promoters that could generate multiple Mitf isoforms with distinct amino-termini, such as ubiquitously expressed Mitf-A and Mitf-H. To gain further insight into Mitf isoform multiplicity and the regulation of the promoter usage of the Mitf gene, we have analyzed the function of the amino-terminal domains of Mitf isoforms and the expression of Mitf mRNA in mouse postnatal testis, which is characterized by spermatogenesis and by a cool temperature because of its unique location. Here we show that the amino-terminal domain of Mitf-A possesses a transactivation activity, as judged by yeast expression analysis. We also show the expression of Mitf-A and Mitf-D mRNAs in testis by PCR-based methods. Moreover, in situ hybridization analysis revealed that an Mitf mRNA, probably representing Mitf-A and/or Mitf-D, is expressed in germ cells, including spermatogonia, spermatocytes that undergo meiosis, and round spermatids with the haploid genome, but is undetectable in elongated spermatids with remodeled and condensed chromatin. Notably, Mitf mRNA is undetectable in somatic Leydig cells and peritubular cells. Therefore, multiple promoters may direct differential expression of the Mitf gene in the testis and contribute to functional diversity of Mitf isoforms.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 134 (1), 143-150, 2003
社団法人 日本生化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204963952768
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- NII論文ID
- 130003534568
- 10012056425
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- NII書誌ID
- AA00694073
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- COI
- 1:CAS:528:DC%2BD3sXnslGlu7g%3D
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- ISSN
- 17562651
- 0021924X
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- NDL書誌ID
- 6640877
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- PubMed
- 12944381
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可