ミューカウント®を用いて分離した小児の<I>Streptococcus mutans</I>の多糖体合成能と齲蝕罹患状況との関係

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  • Relationships between Caries Prevalence and Glucan Synthesizing Ability of <I>Streptococcus mutans</I> isolated from Children by MUCOUNT®

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It is important to have a good grasp of the prevalence of dental caries in a dental caries activity test for children. The Mucount® we used in this study is a testing system for the activity of cariogenic bacterium in saliva. It serves to assess the activity on the basis of the degree of adherence of the cariogenic bacterium. For some reason or other, there are few reports in which the bacterial colonies were identified in a clinical setting. To assess the caries prevalence in children through the caries activity test, we scrutinized the accretion of cariogenic germs by means of the Mucount®The subjects were 62 children who came in for a regular check up at the outpatient clinics of the pedodontics/orthodontics department of our hospital. After isolating S. mutans from the Mucount®wecompared electrophoretic patterns of enzyme-treated DNA. Furthermore, we went over the amount of water-soluble and water-insoluble glucans synthesized by S. mutans classified according to the degree of caries prevalence as well as the amount of glucosyltransferase- I using monoclonal antibodies. A summary of our findings is as follows.<BR>1. The amount of S. mutans collecting on the Mucount® accounted for about one-third of the whole of the isolated amount.<BR>2. A positive correlation was observed between Mucount scores and numbers of df+DF teeth only in the deciduous dentition.<BR>3. The electrophoretic patterns of enzyme-treated DNA of the S. mutans isolated from the oral cavity of the same subject were the same. Between subjects, there were differences of more than 6.56 kbp in electrophoretic pattern.<BR>4. The amount of glucans synthesized by S. mutans and the amount of GTF- I derived from children with the number df+ DF teeth working out at O were significantly smaller than those from children with the caries index exceeding 8.<BR>5. There was no indication that low level of GTF germs induced a high level of GTF germs with the sucrose.<BR>From the above, with the Mucount®scores alone, it was difficult to make an assessment of the prevalence of caries in children. To protect children from caries more effectively, our study suggested, it would be necessary to look into the property of S. mutans inhabiting the oral cavity of each person on an individual basis.

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