CHARACTERISTICS OF INDUCTION OF p27Kip1 AND INVASION INHIBITORY EFFECT OF CEPHARANTHINE ON A HUMAN ORAL SQUAMOUS CELL CARCINOMA CELL LINE

• HARADA Koji

  Second Department of Oral and Maxillofacial Surgery, University of Tokushima School of Dentistry

• BANDO Takashi

  Second Department of Oral and Maxillofacial Surgery, University of Tokushima School of Dentistry

• YOSHIDA Hideo

  Second Department of Oral and Maxillofacial Surgery, University of Tokushima School of Dentistry

• SATO Mitsunobu

  Second Department of Oral and Maxillofacial Surgery, University of Tokushima School of Dentistry

Bibliographic Information

Other Title

• ヒト口腔へん平上皮癌細胞に対するセファランチンのｐ２７｀Ｋｉｐ１´発現誘導と浸潤抑制効果の解析

Abstract

Cepharanthine is a biscoclaurine alkaloid extracted from Stephania cepharantha Hayata, and has been widely used for the treatment of alopecia areata and leukopenia during radiation therapy, and can exert antitumor effects on several human cancer cells. In this study, we examined the mechanism of invasion inhibitory effects by cepharanthine on a human oral squamous cell carcinoma (SCC) cell line (B88). Treatment of B88 cells with cepharanthine (10-20μg/ml) resulted in significant suppression of cell growth. Moreover, induction of p27^Kip1 protein and reduction of cyclin E, Skp2 and c-Myc, were detected by Western blotting. In addition, induction of p27^Kip1 mRNA was observed in cepharanthine-treated B88 cells by real-time RT-PCR. Furthermore, cepharanthine markedly inhibited the migration of B88 cells in a Boyden chamber, and out-growth of them. Interestingly, we detected up-regulation of E-cadherin, integrin α5 and maspin, and down-regulation of Tiaml (tumor invasive and metastasis 1) and MTA1 in cepharanthine-treated B88 cells by Western blotting. These results indicate that cepharanthine may inhibit the invasion of a human oral SCC cell through the pathway of p27^Kip1 induction.

Journal

• Japanese jornal of Head and Neck Cancer

  Japanese jornal of Head and Neck Cancer 29 (1), 197-204, 2003

  Japan Society for Head and Neck Cancer

Keywords

• oral squamous cell carcinoma
• cepharanthine
• invasion inhibitory effect
• p27^Kip1

Details

• CRID: 1390282679706189696
• NII Article ID: 10012172272
• NII Book ID: AN00165234
• DOI: 10.5981/jjhnc1974.29.197
• ISSN: 18839878
• ISSN: 09114335
• Web Site: https://search.jamas.or.jp/link/ui/2004100945
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed