牛挽肉，ポテトサラダおよび野菜のドレッシング和えからの腸管出血性大腸菌Ｏ１５７の検出における培養法，免疫磁気ビーズ，イムノクロマト系簡易キットの有用性の検討

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  • Comparison of Immunomagnetic Separation Method and Immunoassay Procedure for <I>Escherichia coli</I> O157 from Raw Ground Beef, Potato Salad and Tossed Salad
  • ギュウ ヒキニク ポテトサラダ オヨビ ヤサイ ノ ドレッシング アエ カラ ノ チョウカン シュッケツセイ ダイチョウキン O157 ノ ケンシュツ ニ オケル バイヨウホウ メンエキ ジキ ビーズ イムノクロマトケイ カンイ キット ノ ユウヨウセイ ノ ケントウ

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In this study, we compared the performance of a variety of systems to detect Escherichia coli 0157 inoculated into different kinds of foods. Samples were subjected to enrichment culture followed by plating onto sorbitol MacConkey agar (SMAC) or SMAC containing cefixime and potassium tellurite (CT-SMAC) with or without immunomagnetic separation (IMS). Finally E. coli O157 was detected by using commercial immunoassay kits after enrichment culture. The enrichment cultures were done for 18 hr at 42°C, using four different media such as trypticase soy broth (TSB), TSB containing cefixime, tellurite and vancomycin (CTV-TSB), modified EC broth (mEC) and mEC containing novobiocin (N-mEC) for experiment1 (raw ground beef), or using CTV-TSB and N-mEC for experiment 2 (raw ground beef, potato salad and tossed salad). Immunoassays were performed uSing five different commercial kits such as VIP, Reveal, PATH-STIC, EZ Coli and Now E. coli. These experiments were performed simultaneously by six different laboratories with the same materials and methods.<BR>The IMS procedure following enrichment culture increased the detection rate of E. coli O157, irrespective of the enrichment media. E. coli O157 was recovered in 49 and 117 samples out of 144 and 216, respectively, while only 38 and 73 samples were positive when the IMS was not applied, irrespective of experiments 1 and 2. In experiment 1, CTV-TSB showed the best recovery rate among the four enrichment media, while TSB was the only media that recovered the E. coli O157 at a level of 5.5×10-2-5.5×10-4 cfu/25 g. In experiment 2, CTV-TSB showed a better recovery rate than N-mEC from raw ground beef, but the recoveries from potato salad and tossed salad were slightly better in N-mEC than in CTVTSB. The fishing of E. coli O157 from CT-SMAC increased the detection rate of E. coli 0157 in both experiments. From the results we recommend the use of CTV-TSB and CT-SMAC for the detection of E. coli O157 especially in raw ground beef contaminated by competitive bacteria. The immunoassays detected E. coli O157, irrespective of commercial kit used, and the recovery rate was almost as good as culture diagnosis without IMS procedure. The immunoassays used in this study are simple, and the test results are less influenced by individual techniques than the isolation method. They are convenient to use in screening procedure for the detection of E. coli 0157 from large quantities of food samples.

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