Immunohistochemical studies of PIVKA-II in hepatocellular carcinoma by indirect immunofluorescence

• Kuwahara Naoaki

• Higashi Toshihiro

• Nouso Kazuhiro

• Ito Toshio

• Tsuji Takao

抄録

Tissue PIVKA-II was examined in 32 hepatocellular carcinomas and 2 metastatic liver tumors using indirect immunofluorescence, and the results were compared with the size, histological grading and serum PIVKA-II level. The specificity of this method was confirmed by the disappearance of reactivity in PLC/PRF/5 cells after the addition of vitamin K to the culture medium. Positive PIVKA-II staining was observed as a clustered or a single cell pattern only in the HCC nodules, but not in the surrounding cirrhotic tissue. PIVKA-II staining was observed in all HCC groups regardless of histological grade. There was no relationship between PIVKA-II staining and the size of HCC. PIVKA-II was detected immunohistochemically even in small HCC of patients whose plasma PIVKA-II levels were below the detection limit. These results suggest that PIVKA-II production is a specific phenotype of HCC regardless of its histological grading and demonstrate that this immunofluorescent PIVKA-II staining is more sensitive and useful than plasma PIVKA-II assay for the diagnosis of HCC.

収録刊行物

• Acta Medica Okayama

  Acta Medica Okayama 49 (1), 19-24, 1995-02

  Okayama University Medical School

キーワード

• hepatocellular carcinoma
• PIVKA-??immunofluorescent staining
• tumor marker

詳細情報

• CRID: 1390853649527565056
• NII論文ID: 10012275667
• NII論文ID: 120002305279
• NII書誌ID: AA00508441
• ISSN: 0386300X
• DOI: 10.18926/amo/30416
• PubMed: 7762405
• Web Site: https://ousar.lib.okayama-u.ac.jp/30416
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • PubMed
  • CiNii Articles