Apoptosis Induced by Generated OH Radicals Inside Cells after Irradiation.

• HOSOKAWA Yoichiro

  Department of Dental Radiology, Health Sciences University of Hokkaido School of Dentistry

• TANAKA Likinobu

  Department of Dental Radiology, Health Sciences University of Hokkaido School of Dentistry

• KANEKO Masayuki

  Department of Dental Radiology, Health Sciences University of Hokkaido School of Dentistry

• SAKAKURA Yasunori

  Department of Oral Anatomy, Health Sciences University of Hokkaido School of Dentistry

• TSURUGA Eichi

  Department of Oral Anatomy, Health Sciences University of Hokkaido School of Dentistry

• IRIE Kazuharu

  Department of Oral Anatomy, Health Sciences University of Hokkaido School of Dentistry

• YAJIMA Toshihiko

  Department of Oral Anatomy, Health Sciences University of Hokkaido School of Dentistry

抄録

OH radicals play a major role in radiation-induced DNA and cell membrane damage. These types of damage can also induce death by apoptosis through activation of a pro-apoptosis pathway. We attempted to detect OH radicals inside human promyelocytic leukemia (HL60) cells and estimate the relationship between radiation-induced apoptosis and OH radicals generated inside the cells. Electron spin resonance spectroscopy showed that OH radicals were generated by X-rays within irradiated cell pellets and the relative signal intensities of OH radicals increased with the radiation dose. Agarose gel electrophoresis revealed that the death of HL60 cells by apoptosis was accompanied by internucleosomal DNA fragmentation at 2 h after irradiation with 10-30 Gy. On ultrastructure evaluation by transmission electron microscopy, certain irradiated HL60 cells demonstrated condensed chromatin forms at the nuclear membrane and nuclear fragmentation. The frequency of apoptotic cells with condensation and fragmentation of nuclear chromatin increased with radiation dose in semithin sections. The increase of quantitative DNA fragmentation and percentage of non-living cells also correlated with radiation dose. These results suggest that OH radicals are generated inside cells before apoptosis occurs. The amount of OH radicals generated correlates with apoptotic cell death.

収録刊行物

• Ａｒｃｈｉｖｅｓ　ｏｆ　Ｈｉｓｔｏｌｏｇｙ　ａｎｄ　Ｃｙｔｏｌｏｇｙ

  Ａｒｃｈｉｖｅｓ　ｏｆ　Ｈｉｓｔｏｌｏｇｙ　ａｎｄ　Ｃｙｔｏｌｏｇｙ 65 (4), 301-305, 2002

  国際組織細胞学会

キーワード

• Histology

詳細情報

• CRID: 1390001206409638400
• NII論文ID: 10012312802
• NII書誌ID: AA1068990X
• DOI: 10.1679/aohc.65.301
• COI: 1:CAS:528:DC%2BD38Xpslehur8%3D
• ISSN: 13491717
• ISSN: 09149465
• ISSN: http://id.crossref.org/issn/09149465
• PubMed: 12501887
• Web Site: http://www.jstage.jst.go.jp/article/aohc/65/4/65_4_301/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2003126763
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可