B型急性肝炎におけるHBc抗原特異的リンパ球反応の解析 Flowcytometric analysis of HBcAg-specific lymphocyte reaction in acute hepatitis B

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著者

    • 鈴木 貴博 SUZUKI Takahiro
    • 岡山大学大学院医歯学総合研究科 消化器・肝臓・感染症内科学(内科学第一) Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry

抄録

Antigen specific T-cell responeses are important for the eradication of Hepatitis B virus (HBV) in acute infection. Flow cytometric analysis of intracellular cytokines is a simple method for studying the T-cell response at the single-cell level in a short period pf time. In the present syudy, we invesfisated the hepatitis B virus (HBV)-specific T-cell response using flow cytometry. Eight patients with acute hepatitis B and 2 patients with fulminant hepatitis B were studied. Heparinized peripheral blood was incubated with recombinant HB core antigen (HBcAg), and intracytoplasmic cytokines were analyzed by flow cytometry. HBcAg-specific interferon-gamma positive CD4 T-cella were positive in 4 of 10 patients while HBcAg-specific interferon-gamma positive CD8 T-cells and interleukin-4 positive T-cells were negatiive in all patients. No intracytoplasmic cytokines were demonstrated in healthy subjects or with recombinant hepatitis C virus (HCV) antigens. HBcAg-specific CD4 T-cells could be detected by a sensitive flow cytometric analysis of the peripherad blood and the cytokine profile of these cells was Th1. These results suggest that HBcAg-specific Th1-type CD4 T-cells may play an important role in controlling viral clearance during acute HBV infection.

収録刊行物

  • 岡山醫學會雜誌

    岡山醫學會雜誌 114(2), 153-158, 2002-09-30

    岡山医学会

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各種コード

  • NII論文ID(NAID)
    10012336974
  • NII書誌ID(NCID)
    AN00032489
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00301558
  • データ提供元
    CJP書誌  IR 
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