Flowcytometric analysis of HBcAg-specific lymphocyte reaction in acute hepatitis B
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- SUZUKI Takahiro
- Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Bibliographic Information
- Other Title
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- B型急性肝炎におけるHBc抗原特異的リンパ球反応の解析
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Abstract
Antigen specific T-cell responses are important for the eradication of Hepatitis B virus (HBV) in acute infection. Flow cytometric analysis of intracellular cytokines is a simple method for studying the T-cell response at the single-cell level in a short period of time. In the present study, we invesfisated the hepatitis B virus (HBV)-specific T-cell response using flow cytometry. Eight patients with acute hepatitis B and 2 patients with fulminant hepatitis B were studied. Heparinized peripheral blood was incubated with recombinant HB core antigen (HBcAg), and intracytoplasmic cytokines were analyzed by flow cytometry. HBcAg-specific interferon-gamma positive CD4 T-cells were positive in 4 of 10 patients while HBcAg-specific interferon-gamma positive CD8 T-cells and interleukin-4 positive T-cells were negative in all patients. No intracytoplasmic cytokines were demonstrated in healthy subjects or with recombinant hepatitis C virus (HCV) antigens. HBcAg-specific CD4 T-cells could be detected by a sensitive flow cytometric analysis of the peripheral blood and the cytokine profile of these cells was Thl. These results suggest that HBcAg-specific Th1-type CD4 T-cells may play an important role in controlling viral clearance during acute HBV infection.
Journal
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- Okayama Igakkai Zasshi (Journal of Okayama Medical Association)
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Okayama Igakkai Zasshi (Journal of Okayama Medical Association) 114 (2), 153-158, 2002
Okayama Medical Association
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Keywords
Details 詳細情報について
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- CRID
- 1390282679853300224
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- NII Article ID
- 10012336974
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- NII Book ID
- AN00032489
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- ISSN
- 18824528
- 00301558
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- Data Source
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- JaLC
- IRDB
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed