Application of Chicken Immune Sera to Identification of Mycoplasmas by the Filter Immunobinding Method.

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  • TAKAHATA Toshihiro
    Animal Health Products and Chemicals Research Center, Daiichi Pharmaceutical Co.,Ltd.
  • TANIGAWA Masatoshi
    Animal Health Products and Chemicals Research Center, Daiichi Pharmaceutical Co.,Ltd.
  • SHIMIZU Takamasa
    Department of Veterinary Hygiene, Faculty of Agriculture, Miyazaki University

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  • フィルター免疫結合法によるマイコプラズマの同定のための鶏免疫血清の利用
  • Application of Chicken Immune Sera to I

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Abstract

Application of chicken immune sera to identification of Mycoplasma species by the filter immunobinding (FIB) method was investigated. Immune sera against type strains of 15 Mycoplasma species were prepared in chickens by inoculation with concentrated Mycoplasma organisms. Chicken immune sera, when absorbed in a broth medium and diluted in a blocking solution, showed a definite specificity with homologous Mycoplasma antigens by FIB. The identification results of field isolates of Mycoplasma gallisepticum and M. synoviae by FIB using absorbed chicken immune sera were consistent with those obtained by conventional tests based on biochemical properties and growth inhibition using rabbit immune sera. Chicken immune sera were evidenced to be useful for identification of Mycoplasma species when applied to FIB although they were not applicable in growth and metabolism inhibition tests.

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