Electrophoretic Analysis of the Egg Albumen of Jungle Fowl.

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  • ヤケイ卵白蛋白質の電気泳動的解析
  • ヤケイ ランパク タンパクシツ ノ デンキ エイドウテキ カイセキ

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Abstract

Egg albumen of four species of jungle fowl was analyzed by polyacrylamide gel electrophoresis (PAGE) and micro two-dimensional electrophresis (2DE), and compared among those species. The jungle fowl egg albumen was fractionated by PAGE to prealbumen (Pa), ovoalbumen (Ov), conalbumen (TfEW) and ovoglobulin (G2-4).<br>Polymorphisms were recognized in all of the fractions except TfEW. A new variation (C-type) was discovered in the G2 fraction of Gallus lafayettei. In addition, TfEW recognized as an electrophoretic band by PAGE was isolated into three to four continuous spots with 2DE and the existence of isoelectric points with slightly differing molecules was recognized in the TfEW. Three or four clearly-distinct continuous spots in the TfEW were detected exception of Gallus varius.

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