Baculovirus-Mediated Efficient Gene Transfer into the Central Nervous System of the Silkworm, Bombyx mori

この論文にアクセスする

この論文をさがす

著者

抄録

We have successfully developed an efficient gene transfer system using the recombinant baculovirus (AcNPV) in the silkworm, <i>Bombyx mori</i>. A driver-reporter cassette with an <i>EGFP</i> reporter was inserted into AcNPV bacmid using the Bac-to-bac baculovirus expression system. The gene transfer was accomplished by injection of the recombinant AcNPV into larvae or pupae. A cassette driven by the <i>Bombyx actin A3</i> promoter clearly gave EGFP fluorescence in the whole body, including the central nervous system (CNS). To apply this system for analysis of neurohormone gene expression mechanisms, cassettes were constructed with the promoters of three insect neuropeptide hormone genes: <i>bombyxin</i>, a prothoracicotropic hormone (<i>PTTH</i>), and diapause hormone-pheromone biosynthesis activating neuropeptide (<i>DH-PBAN</i>). The <i>bombyxin</i> promoter directed the EGFP expression to the median 8 neurosecretory cells of the brain, <i>PTTH</i> to the lateral 4 neurosecretory cells of the brain and <i>DH-PBAN</i> to 14 cells of the suboesophageal ganglion, which have been identified to be the neuropeptide hormone-producing cells. This assay is time saving, convenient, and highly reproducible, so that the present system provides a new tool for analysis of the neuropeptide hormone gene expression in the CNS.

収録刊行物

  • Journal of insect biotechnology and sericology

    Journal of insect biotechnology and sericology 72(3), 149-155, 2003-10-31

    社団法人 日本蚕糸学会

参考文献:  30件中 1-30件 を表示

被引用文献:  3件中 1-3件 を表示

各種コード

  • NII論文ID(NAID)
    10012453338
  • NII書誌ID(NCID)
    AA11558849
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13468073
  • NDL 記事登録ID
    6730750
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産)
  • NDL 請求記号
    Z54-J696
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ