Simple Manipulation of Silkworm Molting by an Artificial Diet Containing Plant-Derived 20-Hydroxyecdysone

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Last instar larvae of the silkworm, <i>Bombyx mori</i>, were fed an artificial diet containing plant-derived 20-hydroxyecdysone (20E) solution to establish simple recipes for molt manipulation applicable to physiological, biochemical, and molecular biological studies. 20E diet from day 0 or day 1 induced an extra larval molt, while 20E diet from day 2 or thereafter induced precocious pupation, depending on the hemolymph JH titer. We advocate three treatments for stable molt manipulation: (1) rapid induction of an extra larval molt within 2.5 days after feeding 400 ppm of 20E from day 0, (2) late induction of an extra larval molt after feeding 20 ppm from day 0 for 1 week, and (3) 2 days’ acceleration of pupation after feeding 20 ppm from day 4. In the first two treatments, the generated 6th instar larvae eventually died without pupation, while the precocious pupae generated in the third treatment metamorphosed normally into adults. We also measured the hemolymph ecdysteroid titer to clarify the endocrinological environment of 20E-fed larvae.

収録刊行物

  • Journal of insect biotechnology and sericology

    Journal of insect biotechnology and sericology 72(3), 197-201, 2003-10-31

    社団法人 日本蚕糸学会

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各種コード

  • NII論文ID(NAID)
    10012453453
  • NII書誌ID(NCID)
    AA11558849
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13468073
  • NDL 記事登録ID
    6730959
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産)
  • NDL 請求記号
    Z54-J696
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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