Specific Inhibition of Transient and Stable EGFP Gene Expression by Double Stranded RNA Interference in Mouse Preimplantation Embryos

• Hosoe Misa

  National Institute of Agrobiological Sciences

• Furusawa Tadashi

  National Institute of Agrobiological Sciences

• Inoue Fukashi

  National Institute of Agrobiological Sciences Tohoku University

• Sakatani Miki

  National Agricultural Research Center for Kyushu Okinawa Region

• Tokunaga Tomoyuki

  National Institute of Agrobiological Sciences

• Schultz Richard. M.

  University of Pennsylvania

• Takahashi Masashi

  National Agricultural Research Center for Kyushu Okinawa Region

抄録

Double stranded RNA (dsRNA) interference is a useful tool for interfering with gene function by promoting the sequence-dependent degradation of targeted mRNA in several organisms. In the present study, in order to confirm and improve the effect of dsRNA, we investigated an inhibitory effect of dsRNA on both transient and stable gene expression of enhanced green fluorescent protein (EGFP) in mouse preimplantation embryos. In the transient expression system, the rates of fluorescent embryos were significantly decreased by co-injection of EGFP dsRNA and EGFP expression vector fragment into the pronucleus of zygotes. In the stable expression system, EGFP expression in transgenic embryos was significantly decreased by injection of EGFP dsRNA into both the pronucleus and cytoplasm of zygotes, but, cytoplasmic injection caused a more significant EGFP inhibition than pronuclear injection. In quantitative PCR analysis, the expression of the EGFP gene was also inhibited by dsRNA injection, whereas the endogenous gene expression was not affected. These data suggest that dsRNA can inhibit the specific gene expression without affecting the development and expression of other genes.<br>

収録刊行物

• 日本哺乳動物卵子学会誌

  日本哺乳動物卵子学会誌 20 (3), 99-105, 2003

  日本卵子学会

キーワード

• RNAi
• dsRNA
• EGFP
• Gene expression
• Mouse embryos

詳細情報

• CRID: 1390282679314869248
• NII論文ID: 10012455054
• NII書誌ID: AN10548943
• DOI: 10.1274/jmor.20.99
• ISSN: 13475878
• ISSN: 13417738
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可