A Genetic Quality Testing System for Early Stage Embryos in the Mouse

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抄録

We have established a genetic quality testing system for early stage embryos of the mouse. A method of preparation of template DNA for PCR was established using the lysis buffer (1 × PCR reaction buffer supplemented with proteinase K at a concentration of 40 μg/ml) developed by the authors. We demonstrated that two 8-cell embryos of an inbred strain provide sufficient volumes of template DNA for PCR to identify the strain of embryos using four microsatellite markers (<i>D3Mit54</i>, <i>D5Mit18</i>, <i>D6Mit15</i> and <i>D8Mit50</i>) differentiating 13 inbred strains of mice. This system will be useful in embryo banks that have recently been established worldwide for demonstrating the genetic accuracy of a given strain prior to recovery of live animals.<br>

収録刊行物

  • Experimental animals

    Experimental animals 52(5), 397-400, 2003-10-01

    Japanese Association for Laboratory Animal Science

参考文献:  11件中 1-11件 を表示

被引用文献:  2件中 1-2件 を表示

各種コード

  • NII論文ID(NAID)
    10012457984
  • NII書誌ID(NCID)
    AA11032321
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13411357
  • NDL 記事登録ID
    6707214
  • NDL 雑誌分類
    ZS7(科学技術--医学)
  • NDL 請求記号
    Z54-H752
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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