Oxydation of Quercetin by Salivary Components II. Effects of Quercetin on Reactive Oxygen Metabolism by Salivary Polymorphonuclear Leukocytes.

• HIROTA Sachiko

  Department of Nutritional Science, Kyushu Women\\qs University

• TAKAHAMA Umeo

  Kyushu Dental University

• ANSAI Toshihiro

  Kyushu Dental University

• YOSHITAMA Kunijiro

  Department of Biology, Kumamoto University

Abstract

Salivary polymorphonuclear leukocytes (SPMN) produce O₂⁻ and H₂O₂ reducing molecular oxygen, and quercetin is a phenolic compound found in vegetables and fruits. The aim of the present study was to elucidate the interaction between SPMN and quercetin. The oxygen consumption by SPMN, which was stimulated by phorbol myristate acetate (PMA), was suppressed by quercetin. Half-inhibition was observed at about 20 μM quercetin. During inhibition of the oxygen uptake, quercetin was oxidized; the oxidation was inhibited by superoxide dismutase and enhanced by horseradish peroxidase. Quercetin was taken up into SPMN and myeloperoxidase in SPMN could oxidize quercetin if H₂O₂ was provided. These results indicate that quercetin can inhibit the formation of O₂⁻ and H₂O₂ by PMA-stimulated SPMN and that the flavonol can scavenge O₂⁻ and H₂O₂ generated by the SPMN. Quercetin inhibited the growth of Porphyromonas gingivalis that might cause periodontal disease. According to the above results, quercetin seems able to suppress SPMN-dependent inflammation caused by reactive oxygen species inhibiting the growth of bacteria in the mouth.<br>

Journal

• Food Science and Technology Research

  Food Science and Technology Research 8 (3), 276-280, 2002

  Japanese Society for Food Science and Technology

Keywords

• oxygen metabolism
• quercetin
• salivary polymorphonuclear leukocyte
• scavenging of reactive oxygen species

Details

• CRID: 1390282679432630656
• NII Article ID: 10012602634
• NII Book ID: AA11320122
• DOI: 10.3136/fstr.8.276
• COI: 1:CAS:528:DC%2BD38XptlCmsbw%3D
• ISSN: 18813984
• ISSN: 13446606
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed