NMR法によるRNAの立体構造解析における残余双極子相互作用の効果 Effect of Residual Dipolar Coupling Information on the Structure Determination of RNA by NMR

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著者

    • 馬場 清喜 BABA Seiki
    • 千葉工業大学工学部工業化学科 Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology
    • 清宮 恭子 SEIMIYA Kyoko
    • 千葉工業大学工学部工業化学科 Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology
    • 染谷 龍彦 SOMEYA Tatsuhiko
    • 千葉工業大学工学部工業化学科 Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology
    • 坂本 泰一 SAKAMOTO Taiichi
    • 千葉工業大学工学部工業化学科 Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology
    • 河合 剛太 KAWAI Gota
    • 千葉工業大学工学部工業化学科 Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology

抄録

The global conformation of long RNA molecules containing bulge or internal loop are typically not well characterized by conventional NMR methods that rely on NOE-derived short-range distance restraints and J-coupling derived torsion angle restraints. Recently, residual dipolar couplings (RDCs) that provide long-range angular information are being used for structure determination of bio-molecules including RNAs. In this study, RDCs were used for structural determination of a model RNA including bulge, and the effect of RDCs on the structure determination was analyzed. The global structure of the model RNA was improved by the refinement with RDCs. Furthermore, the local structure of the bulge was significantly improved, despite RDCs in the bulge region were not included in the refinement. Thus, it was clearly shown that the RDC refinement was valuable for the structure determination of RNAs containing bulge or internal loop.

収録刊行物

  • 分光研究 = Journal of the spectroscopical research of Japan

    分光研究 = Journal of the spectroscopical research of Japan 53(3), 171-176, 2004-06-15

    The Spectroscopical Society of Japan

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キーワード

各種コード

  • NII論文ID(NAID)
    10012627421
  • NII書誌ID(NCID)
    AN00222531
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00387002
  • NDL 記事登録ID
    7006730
  • NDL 雑誌分類
    ZM35(科学技術--物理学)
  • NDL 請求記号
    Z15-1
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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