Experimental study of injectable bone for regeneration using mesenchymal stem cells, fibrin glue, and a biodegradable scaffold

DOI 18 References
  • OZAWA Ryotaro
    Department of Protective Care for Masticatory Disorders, Field of Head and Neck and Sensory Organ Medicine, Nagoya University, Postgraduate School of Medicine
  • YAMADA Yoichi
    Center for Genetic and Regenerative Medicine, Nagoya University school of Medicine
  • HIBINO Yoshitaka
    Department of Protective Care for Masticatory Disorders, Field of Head and Neck and Sensory Organ Medicine, Nagoya University, Postgraduate School of Medicine
  • NAGASAKA Tetsuro
    Department of Laboratory Medicine, Nagoya University, Postgraduate School of Medicine
  • FU Saisei
    Department of Protective Care for Masticatory Disorders, Field of Head and Neck and Sensory Organ Medicine, Nagoya University, Postgraduate School of Medicine
  • OKAZAKI Yasuhiro
    Department of Protective Care for Masticatory Disorders, Field of Head and Neck and Sensory Organ Medicine, Nagoya University, Postgraduate School of Medicine
  • HATA Ken-ichiro
    Center for Genetic and Regenerative Medicine, Nagoya University school of Medicine
  • UEDA Minoru
    Department of Protective Care for Masticatory Disorders, Field of Head and Neck and Sensory Organ Medicine, Nagoya University, Postgraduate School of Medicine

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  • 間葉系幹細胞，フィブリンのりおよび生体吸収性足場を用いた注入法による骨再生に関する実験的研究

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Abstract

The purpose of this study was to investigate whether a combination of fibrin glue, biodegradable scaffold (β-tricalcium phosphate;β-TCP), and mesenchymal stem cells (MSCs) would promote new bone formation and provide three-dimensional templates for bone growth at heterotopic sites in rats with plasticity. Growing MSCs and developing matrices, explanted from the rat femur, were minced and mixed with fibrin glue in a syringe. The MSCs/fibrin glue-β-TCP complex was injected into the subcutaneous space of the back of the rat. Eight weeks after implantation, macroscopic examination revealed a pearly opalescence that was firm on palpation. Histologic investigations showed newly formed bone structures in all rats treated with the MSCs/fibrin glue-β-TCP complex. No bone structures were found in the fibrin glue or β-TCP control groups. Our results demonstrate that injectable MSCs/fibrin glue-β-TCP complex can provide a three-dimensional scaffold for the successful transplantation and engraftment of osteoblasts. This technique holds promise of becoming a minimally invasive means of delivering autogenous bone to correct or repair bone defects such as those associated with osteoprotic fractures, paradontosis, and tumor resection.

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