ヒト口腔扁平上皮がん細胞株および組織におけるHMGIファミリーの発現 HMGI family expression in human oral squamous carcinoma cell lines and tissues

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著者

    • 宮澤 淳 MIYAZAWA Jun
    • 日本歯科大学大学院歯学研究科口腔外科学1 Department of Oral and Maxillofacial Surgery I, Graduate School of Dentistry, Nippon Dental University
    • 白川 正順 SHIRAKAWA Masayori
    • 日本歯科大学大学院歯学研究科口腔外科学1 Department of Oral and Maxillofacial Surgery I, Graduate School of Dentistry, Nippon Dental University
    • 今井 一志 IMAI Kazushi
    • 日本歯科大学歯学部生化学講座 Department of Biochemistry, School of Dentistry, Nippon Dental University
    • 真田 一男 SANADA Kazuo
    • 日本歯科大学歯学部生化学講座 Department of Biochemistry, School of Dentistry, Nippon Dental University

抄録

Expression of mesenchymal marker genes in carcinoma cells contributes to the progression of neoplasms. The HMGI family, consisting of HMGIC, HMGI, and HMGY, binds to transcription factors in enhanceosomes and regulates mesenchymal cell growth. Although aberrant expression of HMGIC initiates mesenchymal tumor formation, the expression of this marker gene in squamous cell carcinomas of the oral cavity has not been investigated previously. The present study was conducted to analyze expression patterns of the HMGI family in oral carcinomas. Elevated expression of HMGIC on conventional reverse transcription polymerase chain reaction (RT-PCR) was observed only in carcinoma cell lines and tissues but not in their normal counterparts. Quantitative analysis of expression levels by real-time RT-PCR demonstrated that HMGIC is up-regulated in E-cadherin-expressing cell lines. Immunohistochemical studies of carcinoma tissue sections found that HMGIC was localized in E-cadherin-positive carcinoma cells, whereas at the invasion front HMGIC was present in PCNA-positive carcinoma cells located at periphery of carcinoma cell nests and in surrounding fibroblast-like cells. The percentage of HMGIC-positive carcinoma cells decreased in poorly differentiated tumor tissue. The percentage of HMGIC-positive cases at the invasive front significantly increased in patients with cervical lymph node metastasis. These results suggest that HMGIC may have a role in the phenotypic alteration of carcinoma cells and the progression of oral carcinomas.

収録刊行物

  • 日本口腔外科学会雑誌

    日本口腔外科学会雑誌 49(6), 367-375, 2003-06-20

    Japanese Society of Oral and Maxillofacial Surgeons

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キーワード

各種コード

  • NII論文ID(NAID)
    10012629399
  • NII書誌ID(NCID)
    AN00189163
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00215163
  • データ提供元
    CJP書誌  J-STAGE 
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