Simultaneous Measurement of Growth and Movement of Cells Exploiting On-Chip Single-Cell Cultivation Assay

DOI Web Site Web Site Web Site 被引用文献1件 参考文献27件
  • Umehara Senkei
    Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo
  • Hattori Akihiro
    Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo
  • Wakamoto Yuichi
    Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo
  • Yasuda Kenji
    Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo

この論文をさがす

抄録

We have developed an on-chip single-cell microcultivation assay as a means of simultaneously observing the growth and movement of single bacterial cells during long-term cultivation. This assay enables the direct observation of single cells captured in microchambers fabricated on thin glass slides and having semipermeable membrane lids, in which the cells can swim within the space without escape for the long periods. Using this system, the relationship between the cell cycle and the tendency of movement was observed and it was found that the mean free path length did not change during the cell cycle, and that the growth and the swimming were not synchronized. The result indicates that the ability of movement of the cells was independent of the cell cycle.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (27)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ