A Simple and Efficient Method for Somatic Embryogenesis and Plant Regeneration from Leaves of Chrysanthemum [Dendranthema ×grandiflorum (Ramat. Kitamura)]

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A simple and efficient method for somatic embryogenesis of chrysanthemum [Dendranthema × grandiflorum (Ramat.) Kitamura] was established. The best result of somatic embryogenesis was obtained with MS medium containing 2.0 mg l<SUP>-1</SUP> 2, 4-dichlorophenoxyacetic acid and 1.0 mg l<SUP>-1</SUP> kinetin. Embryos with roots 1 to 2 mm long gave the highest frequency for conversion of embryos to plantlets. Root meristems appeared to be formed in somatic embryos by 20 days after culture initiation, while the shoot apices formed after replanting in phytohormone-free medium, resulting in a bipolar structure. The embryo-specific ECP63 gene was expressed in the somatic embryos, as in zygotic embryos, confirming that the plant regeneration occurred via embryogenesis. The growth habits and leaf and flower morphology of the regenerated plantlets were normal, but the day of flowering differed from that of cutting controls. The present somatic embryogenesis method was applicable to 8 out of 13 cultivars studied.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 21(1), 25-33, 2004-03-01

    日本植物細胞分子生物学会

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各種コード

  • NII論文ID(NAID)
    10012727084
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    6884207
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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