Effectiveness of Sonication on Diminishing Bacterial Overgrowth in Agrobacterium-mediated Transformation

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著者

    • Efendi EFENDI
    • Plant Breeding Laboratory, Okinawa Subtropical Station, Japan International Research Center for Agriculture Sciences
    • TAMURA Yasuaki
    • Plant Breeding Laboratory, Okinawa Subtropical Station, Japan International Research Center for Agriculture Sciences
    • TANIO Masahiko
    • Plant Breeding Laboratory, Okinawa Subtropical Station, Japan International Research Center for Agriculture Sciences
    • MATSUOKA Makoto
    • Plant Breeding Laboratory, Okinawa Subtropical Station, Japan International Research Center for Agriculture Sciences

抄録

This study was carried out to trace the biological effects of exposing Agrobacterium tumefaciens and sugarcane cell clumps of suspension culture to ultrasonic waves. A diluted suspension of Agrobacterium tumefaciens, strains EHA101 and LBA4404, was treated with 44 kHz sonication which remarkably reduced the frequency of colony formation in the inoculated medium of both strains. The cell clumps of the suspension culture were co-cultured with Agrobacterium, and then sonicated. Following 3 days repeating co-culture in solid medium, the cell clumps were transferred to the MS-1e medium. Without sonication treatment, 31% of cell clumps revealed bacterial overgrowth, whereas no overgrowth was found in the sonication treatment after 14 days of culture. However, no significant differences in the rates of growth and shoot regeneration between the sonication-treated cell clumps and the untreated cell clumps.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 21(1), 53-56, 2004-03-01

    日本植物細胞分子生物学会

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各種コード

  • NII論文ID(NAID)
    10012727172
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    13424580
  • NDL 記事登録ID
    6884266
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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