Intramolecular Fluorescent Resonance Energy Transfer (FRET) by BODIPY Chemical Modification of Cysteine-engineered Mutants of Green Fluorescent Protein

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著者

    • SUZUKI Miho
    • Department of Functional Materials and Science, Faculty of Engineering, Saitama University
    • ITO Yoichiro
    • Department of Biotechnology, Graduate School of Engineering, Material and Life Science, Osaka University
    • HUSIMI Yuzuru
    • Department of Functional Materials and Science, Faculty of Engineering, Saitama University

抄録

Green Fluorescent Proteins (GFP) bearing an intramolecular BODIPY fluorophore partner were designed by molecular modeling based on GFP's three-dimensional structure, and prepared by mutating out native Cys-48 found at the GFP surface (replaced by Ala or Ser) and introducing new specific, surface cysteine residues for chemical modification by a thiol-directed fluorescent BODIPY 507/545 label. This gave derivatives which exhibited strong intramolecular fluorescence resonance energy transfer with quenching of the emission peak of GFP at 508 nm and the appearance of a new FRET emission from the conjugated BODIPY 507/545 around 530 nm.

収録刊行物

  • Chemistry letters

    Chemistry letters 32(3), 306-307, 2003-03-05

    The Chemical Society of Japan

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各種コード

  • NII論文ID(NAID)
    10012815184
  • NII書誌ID(NCID)
    AA00603318
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    03667022
  • データ提供元
    CJP書誌  J-STAGE 
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