Detection of A/G Single Nucleotide Alteration in RNA Using Base-discriminating Fluorescent Oligodeoxynucleotides

この論文にアクセスする

この論文をさがす

著者

    • OKAMOTO Akimitsu
    • Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University
    • TAINAKA Kazuki
    • Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University
    • SAITO Isao
    • Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University

抄録

We report the fluorescence properties of oligodeoxynucleotides (ODNs) containing a base-discriminating fluorescent nucleoside, benzopyridopyrimidine (BPP), in hybridizing with RNAs possessing different bases opposite BPP. BPP-containing ODN selectively exhibited a strong fluorescence when the RNA base opposite BPP was A, whereas the fluorescence of BPP-containing ODN hybridized with RNA possessing G opposite BPP was very weak. BPP acts as a good base-discriminating fluorescent nucleoside for A/G single base alteration of RNA.

収録刊行物

  • Chemistry letters

    Chemistry letters 32(8), 684-685, 2003-08-05

    The Chemical Society of Japan

参考文献:  6件中 1-6件 を表示

  • <no title>

    COOPER D. N.

    The Metabolic and Molecular Bases of Inherited Disease 1, 259, 1995

    被引用文献1件

  • <no title>

    WANG D. G.

    Science 280, 1077, 1998

    被引用文献4件

  • <no title>

    HAGA H.

    J. Hum. Genet. 47, 605, 2002

    被引用文献1件

  • <no title>

    BLOOMFIELD V. A. ed.

    Nucleic Acids : Structures, Properties, and Functions, 2000

    被引用文献1件

  • <no title>

    CHAKRAVARTI A.

    Nat. Genet. 21(Suppl. 1), 56, 1999

    被引用文献5件

  • <no title>

    OKAMOTO A.

    J. Am. Chem. Soc. 125, 4972, 2003

    被引用文献4件

被引用文献:  2件中 1-2件 を表示

各種コード

  • NII論文ID(NAID)
    10012818078
  • NII書誌ID(NCID)
    AA00603318
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    03667022
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
ページトップへ