Construction of BAC library for the amphibian Xenopus tropicalis

• Ishii Yasuyuki

• Asakawa Shuichi

• Taguchi Yusuke

• Ishibashi Shoko

• Yagi Takeshi

• Shimizu Nobuyoshi

Abstract

Xenopus tropicalis has become an alternative model to the amphibian Xenopus laevis because it is better suited for genetic and genomic studies. We have constructed a genomic BAC library consisting of over 100,000 clones from sperm of Xenopus tropicalis. Analysis by pulsed field gel electrophoresis of representative BAC clones indicated the average size of insert DNA to be 100 kb, and we estimated the library covers 6 times the Xenopus tropicalis genome of 1.7 × 10⁹ base pairs. To evaluate the BAC library, we attempted to isolate BAC clones which contain a protocadherin g (Pcdhg) gene and found that the isolated BAC clones are assembled as two separate contigs. This result suggests the presence of at least two clusters for the Pcdhg gene in the genome of X. tropicalis. <br>

Journal

• Genes & Genetic Systems

  Genes & Genetic Systems 79 (1), 49-51, 2004

  The Genetics Society of Japan

Keywords

• amphibian
• BAC
• genomic library
• <i>Pcdh</i> γ
• <i>Xenopus tropicalis</i>

Details

• CRID: 1390001205474391680
• NII Article ID: 10012907202
• NII Book ID: AA11077421
• DOI: 10.1266/ggs.79.49
• ISSN: 18805779
• ISSN: 13417568
• NDL BIB ID: 6895575
• PubMed: 15056936
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6895575
• Web Site: http://www.jstage.jst.go.jp/article/ggs/79/1/79_1_49/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed