糖クラスターを用いるジーンデリバリー : ウイルスサイズ(50nm)を目指して Gene Delivery Using Glycoclusters : Designing Viral (50nm) Sizes
Endocytosis is a drinking activity of eukaryotic cells, which thereby ingest a part of their plasma membrane to form endocytic vesicles (≤100 nm), into which external objects such as viruses are swallowed. It is of an utmost importance to control the size of artificial molecular (gene, drug, and probe) delivery machines in this viral size range. A 7040 base-pair plasmid DNA pCMVluc can be stoichiometrically coated with 5 nm sized glycocluster nanoparticles (GNPs) formed by micellar aggregation of calix  resorcarene-based glycocluster amphiphile to give compactly packed and <I>monomeric</I> glycoviruses of a size of 40-50 nm. The lactose-functionalized neutral virus thus obtained can efficiently transfect hepatic cells via receptor-mediated endocytosis without any notable cytotoxicity. It works <I>in vivo</I> (mouse), too. We also paid attention to the endocytosis susceptibilities of GNP (5 nm) as well as a 15 nm sized, saccharide-coated quantum dot species, in light of that of glycovirus. The dramatic decrease in the activities with decreasing sizes reveals an optimal size at around 50 nm. The size selectivity thus arises from size complementarity in the encapsulation of nanometric guest particles by endocytic vesicles as a macrobiomolecular host.
- 有機合成化学協会誌 : JOURNAL OF Synthetic Organic Chemistry JAPAN
有機合成化学協会誌 : JOURNAL OF Synthetic Organic Chemistry JAPAN 62(5), 529-533, 2004-05-01
The Society of Synthetic Organic Chemistry, Japan