糖クラスターを用いるジーンデリバリー : ウイルスサイズ(50nm)を目指して Gene Delivery Using Glycoclusters : Designing Viral (50nm) Sizes

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Endocytosis is a drinking activity of eukaryotic cells, which thereby ingest a part of their plasma membrane to form endocytic vesicles (≤100 nm), into which external objects such as viruses are swallowed. It is of an utmost importance to control the size of artificial molecular (gene, drug, and probe) delivery machines in this viral size range. A 7040 base-pair plasmid DNA pCMVluc can be stoichiometrically coated with 5 nm sized glycocluster nanoparticles (GNPs) formed by micellar aggregation of calix [4] resorcarene-based glycocluster amphiphile to give compactly packed and <I>monomeric</I> glycoviruses of a size of 40-50 nm. The lactose-functionalized neutral virus thus obtained can efficiently transfect hepatic cells via receptor-mediated endocytosis without any notable cytotoxicity. It works <I>in vivo</I> (mouse), too. We also paid attention to the endocytosis susceptibilities of GNP (5 nm) as well as a 15 nm sized, saccharide-coated quantum dot species, in light of that of glycovirus. The dramatic decrease in the activities with decreasing sizes reveals an optimal size at around 50 nm. The size selectivity thus arises from size complementarity in the encapsulation of nanometric guest particles by endocytic vesicles as a macrobiomolecular host.

収録刊行物

  • 有機合成化学協会誌 : JOURNAL OF Synthetic Organic Chemistry JAPAN

    有機合成化学協会誌 : JOURNAL OF Synthetic Organic Chemistry JAPAN 62(5), 529-533, 2004-05-01

    The Society of Synthetic Organic Chemistry, Japan

参考文献:  9件中 1-9件 を表示

  • <no title>

    ALBERTS B.

    Molecular Biology of Cell Chapter 13, 746, 2002

    被引用文献1件

  • <no title>

    FUJIMOTO T.

    J. Am. Chem. Soc. 119, 6676, 1997

    被引用文献6件

  • <no title>

    FUJIMOTO T.

    J. Am. Chem. Soc. 120, 601, 1998

    被引用文献8件

  • <no title>

    FUJIMOTO K.

    J. Am. Chem. Soc. 122, 3585, 2000

    被引用文献1件

  • <no title>

    HAYASHIDA O.

    J. Am. Chem. Soc. 125, 601, 2003

    被引用文献1件

  • <no title>

    AOYAMA Y.

    Chem. Eur. J. 10, 588, 2004

    被引用文献1件

  • <no title>

    NAKAI T.

    J. Am. Chem. Soc. 125, 8465, 2003

    被引用文献2件

  • <no title>

    HAYASHIDA O.

    J. Am. Chem. Soc. 121, 11597, 1999

    DOI 被引用文献4件

  • <no title>

    AOYAMA Y.

    J. Am. Chem. Soc. 125, 3455, 2003

    被引用文献2件

各種コード

  • NII論文ID(NAID)
    10012930295
  • NII書誌ID(NCID)
    AN0024521X
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00379980
  • NDL 記事登録ID
    6946204
  • NDL 雑誌分類
    ZP11(科学技術--化学・化学工業--有機化学・有機化学工業)
  • NDL 請求記号
    Z17-256
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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