Broad Specificity in Binding of NIPP-1, Nuclear Inhibitor of Protein Phosphatase-1, to PP1 Isoforms in Vivo
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- Kim Sei-Eun
- Division of Biochemical Oncology and Immunology, Institute for Genetic Medicine, Hokkaido University
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- Shima Hiroshi
- Division of Biochemical Oncology and Immunology, Institute for Genetic Medicine, Hokkaido University
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- Nakamura Koji
- Division of Biochemical Oncology and Immunology, Institute for Genetic Medicine, Hokkaido University
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- Kikuchi Kunimi
- Division of Biochemical Oncology and Immunology, Institute for Genetic Medicine, Hokkaido University
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Abstract
Protein phosphatase type-1 (PP1), one of the most abundant Ser/Thr protein phosphatases, plays a central role in the regulation of various cell functions. Almost all the PP1 molecules exist as holoenzymes in vivo consisting of a catalytic subunit (PP1C) and a variable regulatory subunit that regulates substrate specificity and/or subcellular localization. In order to clarify fine regulation of PP1, we overexpressed a nuclear inhibitor of PP1 (NIPP-1) in a Flag-tagged form in mammalian cells. The Flag-tagged NIPP-1 was found to be immunoprecipitated with three isoforms of PP1C, namely, PP1α, PP1γ1, and PP1δ with a similar efficiency, suggesting that NIPP-1 makes a complex with the PP1C through the region conserved among the three isoforms. These results suggested that NIPP-1 can be involved in the regulation of various PP1 holoenzymes in vivo.
Journal
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- The Tohoku Journal of Experimental Medicine
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The Tohoku Journal of Experimental Medicine 191 (1), 39-45, 2000
Tohoku University Medical Press
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Details 詳細情報について
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- CRID
- 1390001204241402624
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- NII Article ID
- 10013014995
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- NII Book ID
- AA00863920
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- COI
- 1:CAS:528:DC%2BD3cXltFOmt70%3D
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- ISSN
- 13493329
- 00408727
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- PubMed
- 10896038
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed