Efficient plant regeneration and micropropagation from callus derived from mature zygotic embryo of Larix gmelinii

この論文にアクセスする

この論文をさがす

著者

抄録

Calli derived from mature zygotic embryos and seedling explants of Larix gmelinii were used to investigate for organogenesis. After 4 weeks of culture on half-strength DCR medium containing BA, numerous adventitious buds formed on the callus tissues which were derived from zygotic embryos, but none formed on calli generated from seedling explants. Rates of callus proliferation and adventitious bud formation were higher when the callus was transferred to medium supplemented with activated charcoal. To induce shoot elongation, multiple shoots were separated and transferred to medium with 0.05% activated charcoal. To induce adventitious root formation, elongated shoots of 1.5-2.0 cm in length were cultured in half-strength MS medium containing 0.3 mg l<SUP>-1</SUP> NAA and 0.3 mg l<SUP>-1</SUP> IBA. To assess genetic homogeneity and somatic variation, chromosomes of regenerated plantlets were observed. No numerical or structural changes to the chromosomes were found in regenerated plantlets using this system.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 21(2), 159-163, 2004-06-01

    日本植物細胞分子生物学会

参考文献:  20件中 1-20件 を表示

各種コード

  • NII論文ID(NAID)
    10013103227
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    13424580
  • NDL 記事登録ID
    6960309
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ