Separation of Flavonoids by Semi-Micro High-Performance Liquid Chromatography with Electrochemical Detection

DOI PDF Web Site 3 Citations 47 References
  • Jin Dongri
    Chemistry Department of Science & Engineering College, Yanbian University Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science
  • Hakamata Hideki
    Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science
  • Takahashi Kouji
    Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science
  • Kotani Akira
    Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science
  • Kusu Fumiyo
    Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science

Search this article

Abstract

A highly sensitive and selective method was developed for the determination of 15 flavonoids by high-performance liquid chromatography with electrochemical detection (HPLC–ECD) using a microbore column. The 15 flavonoids were divided into two groups according to their hydrophobicity and were resolved by two isocratic systems, namely, methanol–water (1:1 and 3:7, v/v) containing 0.5% phosphoric acid. The retention factor (k) of each flavonoid linearly correlated with the logarithmic partition of coefficients between 1-octanol–water (log P) values. The detection limits (S/N = 3) of the flavonoids tested were in the range of 2–25 fmol; that is 600 times more sensitive than conventional HPLC with UV detection. The relative standard deviations (RSD, n = 5) were less than 5% at 1 pmol for the flavonoids tested. By the present method, only 100 μL of bottled Japanese green tea were enough to determine the flavonoid contents.

Journal

Citations (3)*help

See more

References(47)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top