消化管上皮細胞の容積調節機構 : Clチャネルの役割 Cell volume regulation in intestinal epithelial cells : Role of Cl^- channels

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著者

    • 眞鍋 健一 MANABE Ken-ichi
    • 自然科学研究機構 生理学研究所 機能協関部門 Department of Cell Physiology, National Institute for Physiological Sciences
    • 岡田 泰伸 OKADA Yasunobu
    • 自然科学研究機構 生理学研究所 機能協関部門 Department of Cell Physiology, National Institute for Physiological Sciences

抄録

Cell volume regulation under osmotic stress is one of the important cell functions to maintain cell homeostasis. Absorptive intestinal epithelial cells are obliged to swell in association with active absorption of nutrients, whereas secretory cells in the intestinal crypt exhibit shrinkage due to Na<SUP>+</SUP> and H<SUB>2</SUB>O secretion driven by active Cl<SUP>-</SUP> secretion. After osmotic swelling and shrinkage, intestinal epithelial cells undergo a regulatory volume decrease (RVD) or a regulatory volume increase (RVI), respectively, to restore their original volume. In human epithelial Intestine 407 cells, we demonstrated that the RVD is accomplished by parallel activation of Ca<SUP>2+</SUP>-activated K<SUP>+</SUP> channel (IK1) and volume-sensitive outwardly rectifying Cl<SUP>-</SUP> channel (VSOR). Small intestinal and colonic epithelial cells have been reported to respond with shrinkage to secretagogue stimulation. We utilized a human colonic epithelial cell line, T84, as a model to clarify the ion transport mechanisms of volume responses to secretagogue situmulation. T84 cells exhibited shrinkage, called a secretory volume decrease (SVD), after stimulation with carbachol (CCh), and they thereafter restored their original volume even during CCh stimulation. Our data showed that CCh-induced SVD is induced by Cl<SUP>-</SUP> secretion via Ca<SUP>2+</SUP>-activated Cl<SUP>-</SUP> channels, and, on the other hand, the succeeding RVI is by NaCl uptake via Na<SUP>+</SUP>-K<SUP>+</SUP>-2Cl<SUP>-</SUP> cotransporters (NKCC). These findings indicate that when intestinal cells are faced with osmotic stress, which is inevitably coupled to active absorption and secretion, they can regulate their volume by activating ion channels and/or transporters.

収録刊行物

  • 膜 29(3), 140-147, 2004-05-01

    日本膜学会

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各種コード

  • NII論文ID(NAID)
    10013114357
  • NII書誌ID(NCID)
    AN0023215X
  • 本文言語コード
    JPN
  • 資料種別
    REV
  • ISSN
    03851036
  • NDL 記事登録ID
    6970892
  • NDL 雑誌分類
    ZR2(科学技術--生物学--生化学)
  • NDL 請求記号
    Z18-1127
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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