Preparation of Selenium-enriched Sprouts and Identification of Their Selenium Species by High-performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry

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著者

    • Kondo Mariko KONDO Mariko
    • Laboratory of Food and Nutritional Sciences, Department of Biotechnology, Faculty of Engineering, Kansai University
    • YUJI Mami
    • Laboratory of Food and Nutritional Sciences, Department of Biotechnology, Faculty of Engineering, Kansai University
    • HATTORI Hiroyuki
    • Laboratory of Food and Nutritional Sciences, Department of Biotechnology, Faculty of Engineering, Kansai University
    • YOSHIDA Munehiro
    • Laboratory of Food and Nutritional Sciences, Department of Biotechnology, Faculty of Engineering, Kansai University

抄録

  Sprouts of several plants (10 families and 28 species) were cultivated in a high selenium environment, and the chemical species of selenium in these selenium-enriched sprouts were identified by using high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICPMS). Cultivation of sprouts of kaiware daikon (type of radish) with 5.0 μg/ml or 10.0 μg/ml of selenium as selenite inhibited the growth. However, no abnormalities in the shape or color were apparent even in the sprouts exposed to 10.0 μg/ml of selenium. The selenium concentration in the sprouts of most plants examined was higher than that from environmental exposure. Among the types of selenium that were accumulated, a large part (69–98%) was extractable in 0.2 <small>M</small> HCl. Chemical analysis of selenium in the HCl extract showed that the main selenium species in all the sprouts examined was <I>Se</I>-methylselenocysteine. In addition to <I>Se</I>-methylselenocysteine, selenomethionine, non-metabolized selenite, γ-glutamyl-<I>Se</I>-methylselenocysteine and an unknown selenium compound were also detected in several high-selenium sprouts. Since higher anticarcinogenic activities of these monomethylated selenoamino acids have been observed, it is anticipated that such selenium-enriched sprouts will be used as a foodstuff for cancer prevention.

収録刊行物

  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 68(1), 193-199, 2004-01-23

    公益社団法人 日本農芸化学会

参考文献:  17件中 1-17件 を表示

被引用文献:  4件中 1-4件 を表示

各種コード

  • NII論文ID(NAID)
    10013141274
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    6832737
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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