Introduction of DPR, an Enterostatin Fragment Peptide, into Soybean β-Conglycinin α' Subunit by Site-directed Mutagenesis

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  DPR, a fragment peptide of enterostatin (VPDPR) having hypocholesterolemic activity, was introduced into the three homologous sites, EPR, DYR, and DPI, in the soybean β-conglycinin α′ subunit by site-directed mutagenesis. The modified β-conglycinin was expressed in <I>Escherichia coli</I> and recovered in the soluble fraction. After purification on ion-exchange HPLC, the modified β-conglycinin was digested by trypsin to release integrated DPR. The yield of DPR from 1 mole of the modified β-conglycinin was 1.2 mole.

収録刊行物

  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 68(1), 253-256, 2004-01-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10013141490
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    09168451
  • NDL 記事登録ID
    6832958
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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