Cloning and Sequencing of the ura3 and ura5 Genes, and Isolation and Characterization of Uracil Auxotrophs of the Fungus Mortierella alpina 1S-4
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- Takeno Seiki TAKENO Seiki
- Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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- Sakuradani Eiji SAKURADANI Eiji
- Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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- Murata Shoichi [他] MURATA Shoichi
- Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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- INOHARA (OCHIAI) Misa
- Institute for Advanced Technology, Suntory Ltd.
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- KAWASHIMA Hiroshi
- Institute for Food & Beverage Health Care Science Laboratory, Suntory Ltd.
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- ASHIKARI Toshihiko
- Institute for Advanced Technology, Suntory Ltd.
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- SHIMIZU Sakayu
- Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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著者
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- Takeno Seiki TAKENO Seiki
- Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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- Sakuradani Eiji SAKURADANI Eiji
- Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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- Murata Shoichi [他] MURATA Shoichi
- Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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- INOHARA (OCHIAI) Misa
- Institute for Advanced Technology, Suntory Ltd.
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- KAWASHIMA Hiroshi
- Institute for Food & Beverage Health Care Science Laboratory, Suntory Ltd.
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- ASHIKARI Toshihiko
- Institute for Advanced Technology, Suntory Ltd.
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- SHIMIZU Sakayu
- Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
抄録
The oil-producing fungus <I>Mortierella alpina</I> 1S-4 is an industrial strain. In order to prepare host strains for a transformation system for this fungus, six uracil auxotrophs were obtained by means of random mutation with <I>N</I>-methyl-<I>N</I>′-nitro-<I>N</I>-nitrosoguanidine (MNNG). When the activities of orotate phosphoribosyl transferase (OPRTase, EC 2.4.2.10) and orotidine-5′-phosphate decarboxylase (OMPdecase, EC 4.1.1.23) were examined in the mutants and wild strain, OPRTase activity was found to be completely absent in all mutants, on the other hand, OMPdecase activity was intact. The genomic DNA and cDNA of the <I>ura5</I> gene encoding OPRTase and the <I>ura3</I> gene encoding OMPdecase were cloned and sequenced. The Ura5p deduced amino acid sequence of this fungus showed highest similarity to that of <I>Vibrio cholerae</I> classed among prokaryote. Furthermore, the mutational points in the <I>ura5</I> genes of two selected mutants were identified; a base-replacement and a base-insertion.
収録刊行物
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- Bioscience, biotechnology, and biochemistry
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Bioscience, biotechnology, and biochemistry 68(2), 277-285, 2004-02-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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