Cloning and Sequencing of the ura3 and ura5 Genes, and Isolation and Characterization of Uracil Auxotrophs of the Fungus Mortierella alpina 1S-4

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著者

    • Takeno Seiki TAKENO Seiki
    • Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
    • Sakuradani Eiji SAKURADANI Eiji
    • Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
    • Murata Shoichi [他] MURATA Shoichi
    • Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
    • SHIMIZU Sakayu
    • Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

抄録

The oil-producing fungus <I>Mortierella alpina</I> 1S-4 is an industrial strain. In order to prepare host strains for a transformation system for this fungus, six uracil auxotrophs were obtained by means of random mutation with <I>N</I>-methyl-<I>N</I>′-nitro-<I>N</I>-nitrosoguanidine (MNNG). When the activities of orotate phosphoribosyl transferase (OPRTase, EC 2.4.2.10) and orotidine-5′-phosphate decarboxylase (OMPdecase, EC 4.1.1.23) were examined in the mutants and wild strain, OPRTase activity was found to be completely absent in all mutants, on the other hand, OMPdecase activity was intact. The genomic DNA and cDNA of the <I>ura5</I> gene encoding OPRTase and the <I>ura3</I> gene encoding OMPdecase were cloned and sequenced. The Ura5p deduced amino acid sequence of this fungus showed highest similarity to that of <I>Vibrio cholerae</I> classed among prokaryote. Furthermore, the mutational points in the <I>ura5</I> genes of two selected mutants were identified; a base-replacement and a base-insertion.

収録刊行物

  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 68(2), 277-285, 2004-02-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

参考文献:  39件中 1-39件 を表示

被引用文献:  2件中 1-2件 を表示

各種コード

  • NII論文ID(NAID)
    10013141610
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    6868290
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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