Lack of an Inducible Effect of Dietary Soy Isoflavones on the mRNA Abundance of Hepatic Cytochrome P-450 Isozymes in Rats

• KISHIDA Taro

  Department of Biological Resources, Faculty of Agriculture, Ehime University

• NAGAMOTO Manabu

  Department of Biological Resources, Faculty of Agriculture, Ehime University

• OHTSU Yohhei

  Department of Biological Resources, Faculty of Agriculture, Ehime University

• WATAKABE Miho

  Department of Biological Resources, Faculty of Agriculture, Ehime University

• OHSHIMA Daisuke

  Department of Biological Resources, Faculty of Agriculture, Ehime University

• NASHIKI Kunitaka

  Department of Biological Resources, Faculty of Agriculture, Ehime University

• MIZUSHIGE Takafumi

  Department of Biological Resources, Faculty of Agriculture, Ehime University

• IZUMI Tohru

  Research and Development Division, Kikkoman Corporation

• OBATA Akio

  Research and Development Division, Kikkoman Corporation

• EBIHARA Kiyoshi

  Department of Biological Resources, Faculty of Agriculture, Ehime University

抄録

Modulation of the activity and content of cytochrome P-450 (CYP) in hepatic microsomes may be important to human health since these enzymes activate and inactivate a wide range of xenobiotics and food components. Regulation of the inducibility of most CYPs involves transcriptional regulation and post-transcriptional mRNA stabilization. We examined in the present study the effect of dietary soy isoflavone (0–300 mg of isoflavone/kg of diet) on the mRNA abundance of rat hepatic CYP1A1, CYP1A2, CYP2B1/2, CYP2C11, CYP2E1, CYP3A1, CYP3A2 and CYP4A1 by quantitative competitive RT-PCR and real-time monitored RT-PCR. A fermented soy extract containing 155 mg/g of genistein, 127 mg/g of daidzein, and other minor isoflavones was used as the isoflavone source. The dietary soy isoflavone had no affect on the hepatic mRNA abundance of these CYPs. The results by both methods were well matched and indicate that the dietary soy isoflavone did not cause the induction of CYPs by transcriptional step-up regulation or post-transcriptional mRNA stabilization.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 68 (3), 508-515, 2004

  公益社団法人 日本農芸化学会

キーワード

• soy isoflavone
• mRNA abundance
• cytochrome P450
• rat

詳細情報

• CRID: 1390282681450368896
• NII論文ID: 10013142524
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.68.508
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 6904096
• PubMed: 15056880
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6904096
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.68.508
• Web Site: https://search.jamas.or.jp/link/ui/2006192602
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可