Functional Analysis of Two Processed Fragments of Bacillus thuringiensis Cry11A Toxin
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- YAMAGIWA Masashi
- Department of Bioscience and Biotechnology, Okayama University
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- SAKAGAWA Kohei
- Department of Bioscience and Biotechnology, Okayama University
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- SAKAI Hiroshi
- Department of Bioscience and Biotechnology, Okayama University
Bibliographic Information
- Other Title
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- Functional Analysis of Two Processed Fragments of<i>Bacillus thuringiensis</i>Cry11A Toxin
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Abstract
The 70-kDa protoxin of Cry11A, a dipteran-specific insecticidal protein, was processed by trypsin into 36- and 32-kDa fragments. To investigate the potent function of the two processed fragments, a GST (Glutathione-S-transferase) fusion protein of each polypeptide was constructed. While neither the 36- nor the 32-kDa fragment was toxic to Culex pipiens larvae, coexpression of the two fragments restored the insecticidal activity. Furthermore, the coprecipitation experiment demonstrated that the 36-kDa fragment was associated with the 32-kDa fragment. It was, therefore, shown that the coexistence of the two processed fragments of Cry11A was essential for the toxicity. The mutant of the 36-kDa fragment lacking the region from Gly257 to Arg360 bound to the 32-kDa fragment but the coexpression with the 32-kDa fragment resulted in no toxicity, suggesting that this region was involved in insecticidal activity.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 68 (3), 523-528, 2004
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206473662208
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- NII Article ID
- 10013142578
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 6904117
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- PubMed
- 15056882
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed