DNA Microarray Analysis of Plastid Gene Expression in an<i>Arabidopsis</i>Mutant Deficient in a Plastid Transcription Factor Sigma, SIG2

  • NAGASHIMA Akitomo
    Laboratory of Molecular Genetics, Department of Molecular Biology, Institute of Molecular and Cellular Biosciences, The University of Tokyo
  • HANAOKA Mitsumasa
    Laboratory of Molecular Genetics, Department of Molecular Biology, Institute of Molecular and Cellular Biosciences, The University of Tokyo
  • MOTOHASHI Reiko
    Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center
  • SEKI Motoaki
    Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center
  • SHINOZAKI Kazuo
    Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center
  • KANAMARU Kengo
    Laboratory of Molecular Genetics, Department of Molecular Biology, Institute of Molecular and Cellular Biosciences, The University of Tokyo
  • TAKAHASHI Hideo
    Laboratory of Molecular Genetics, Department of Molecular Biology, Institute of Molecular and Cellular Biosciences, The University of Tokyo
  • TANAKA Kan
    Laboratory of Molecular Genetics, Department of Molecular Biology, Institute of Molecular and Cellular Biosciences, The University of Tokyo

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タイトル別名
  • DNA Microarray Analysis of Plastid Gene Expression in an Arabidopsis Mutant Deficient in a Plastid Transcription Factor Sigma, SIG2

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The plastid genome of higher plants contains more than one hundred genes for photosynthesis, gene expression, and other processes. Plastid transcription is done by two types of RNA polymerase, PEP and NEP. PEP is a eubacteria-type RNA polymerase that is essential for chloroplast development. In Arabidopsis thaliana, six sigma factors (SIG1-6) are encoded by the nuclear genome, and postulated to determine the transcription specificity of PEP. In this study, we constructed a DNA microarray for all of the plastid protein-coding genes, and analyzed the effects of the sig2 lesion on the global plastid gene expression. Of the 79 plastid protein genes, it was found that only the psaJ transcript was decreased in the mutant, whereas transcripts of 47 genes were rather increased. Since many of the up-regulated genes are under the control of NEP, it was suggested that the NEP activity was increased in the sig2-1 mutant.

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