RSC Nucleosome-remodeling Complex Plays Prominent Roles in Transcriptional Regulation throughout Budding Yeast Gametogenesis

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Author(s)

    • MIYAHARA Kohji
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • HAYASIDA Yasufumi
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • TSUCHIYA Eiko
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

Abstract

RSC is a nucleosome-remodeling complex of <I>Saccharomyces cerevisiae</I> essential for growth that can alter histone–DNA interaction by using the energy of ATP hydrolysis. Nps1p/Sth1p is an ATPase subunit of RSC. A mutation in the conserved ATPase domain of Nps1p causes a sporulation defect with decreased expression of early meiotic genes, especially <I>IME2</I>. This defect is partially suppressed by the overexpression of either <I>IME1</I> or <I>IME2</I>. A homozygous diploid of a novel temperature-sensitive <I>nps1</I> mutation, <I>nps1-13</I>, harboring amino acid substitutions within the bromodomain, was unable to sporulate. Overexpression of <I>IME</I>, <I>IME2</I>, or both of these genes allowed the completion of meiosis I and meiosis II in <I>nps1-13</I> but not the formation of mature asci. In <I>nps1-13</I> carrying YEp<I>IME1</I>, the expression of a group of sporulation-specific genes, which express at the middle stages of sporulation and are required for spore-wall formation, notably diminished, and several late sporulation genes expressed at the early stages of sporulation. These results suggest that Nps1p/RSC plays important roles during the spore development process by controlling gene expression for initiating both meiosis and spore morphogenesis, and ensures proper expression timing of late meiotic genes.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 68(4), 909-919, 2004-04-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  45

Codes

  • NII Article ID (NAID)
    10013144047
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09168451
  • NDL Article ID
    6930440
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
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