In Vivo Bioconversion of Tetrahydroisoquinoline by Recombinant Coclaurine N-Methyltransferase

• MORISHIGE Takashi

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

• CHOI Kum-Boo

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

• SATO Fumihiko

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

Bibliographic Information

Other Title

• <i>In Vivo</i>Bioconversion of Tetrahydroisoquinoline by Recombinant Coclaurine<i>N</i>-Methyltransferase

Abstract

Coclaurine N-methyltransferase from Coptis japonica catalyzes the N-methylation of coclaurine as well as simple tetrahydroisoquinoline. We examined the possibility of converting 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline into its N-methylated product using transgenic Escherichia coli, which expressed recombinant coclaurine N-methyltransferase, without the addition of a methyl-group donor. Transgenic E. coli successfully N-methylated the substrate added to the medium and excreted the product. Limitation of bioconversion by the supply of methyl-group donor is discussed.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 68 (4), 939-941, 2004

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• bioconversion
• <I>N</I>-methyltransferase
• tetrahydroisoquinoline

Details

• CRID: 1390282681450465920
• NII Article ID: 10013144166
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.68.939
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 6930488
• PubMed: 15118328
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6930488
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.68.939
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed