In Vivo Bioconversion of Tetrahydroisoquinoline by Recombinant Coclaurine N-Methyltransferase
Coclaurine <I>N</I>-methyltransferase from <I>Coptis japonica</I> catalyzes the <I>N</I>-methylation of coclaurine as well as simple tetrahydroisoquinoline. We examined the possibility of converting 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline into its <I>N</I>-methylated product using transgenic <I>Escherichia coli</I>, which expressed recombinant coclaurine <I>N</I>-methyltransferase, without the addition of a methyl-group donor. Transgenic <I>E. coli</I> successfully <I>N</I>-methylated the substrate added to the medium and excreted the product. Limitation of bioconversion by the supply of methyl-group donor is discussed.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 68(4), 939-941, 2004-04-23