Substrate Specificity of Aminopeptidase from the Mid-gut Gland of the Scallop (<i>Patinopecten yessoensis</i>)

• UMETSU Hironori

  Graduate School of Environmental Science, Aomori University Institute of Bioscience and Biotechnology, Aomori University

• ARAI Mito

  Graduate School of Environmental Science, Aomori University

• OTA Toshinori

  Institute of Bioscience and Biotechnology, Aomori University

• ABE Kaoru

  Aomori Industrial Research Center

• UCHIZAWA Hidemitsu

  Aomori Industrial Research Center

• SASAKI Kazuo

  Institute of Bioscience and Biotechnology, Aomori University

書誌事項

タイトル別名

• Substrate Specificity of Aminopeptidase from the Mid-gut Gland of the Scallop (Patinopecten yessoensis)

抄録

An action for various peptides and a kinetic study for amino acid p-nitroanilides (pNAs) and 4-methylcoumaryl-7-amides (MCAs) were performed with purified aminopeptidase from the mid-gut of the scallop. The enzyme preferred dipeptides having Ala, Met, and Phe in the amino-terminal or the penultimate position from the amino-termini. The catalytic efficiencies, k_cat⁄K_m values for Ala-pNA and MCA were the highest in the tested substrates, and those for pNA and MCA substrates having Met or Phe were the next highest. The enzyme was found to be a new alanine-specific aminopeptidase.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 68 (4), 945-947, 2004

  公益社団法人 日本農芸化学会

キーワード

• scallop
• mid-gut gland
• aminopeptidase
• substrate specificity
• kinetic parameter

詳細情報

• CRID: 1390001206473656064
• NII論文ID: 10013144184
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.68.945
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 6930512
• PubMed: 15118330
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6930512
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.68.945
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可