Effect of Ethanol on Cell Growth of Budding Yeast : Genes That Are Important for Cell Growth in the Presence of Ethanol

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著者

    • Takeo Ikuko TAKEO Ikuko
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • KANAI Muneyoshi
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • SHITAMUKAI Atsunori
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • MIZUNUMA Masaki
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • MIYAKAWA Tokichi
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • SHIMOI Hitoshi
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • HIRATA Dai
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

抄録

The budding yeast <I>Saccharomyces cerevisiae</I> has been used in the fermentation of various kinds of alcoholic beverages. But the effect of ethanol on the cell growth of this yeast is poorly understood. This study shows that the addition of ethanol causes a cell-cycle delay associated with a transient dispersion of F-actin cytoskeleton, resulting in an increase in cell size. We found that the tyrosine kinase Swe1, the negative regulator of Cdc28-Clb kinase, is related to the regulation of cell growth in the presence of ethanol. Indeed, the increase in cell size due to ethanol was partially abolished in the <I>SWE1</I>-deleted cells, and the amount of Swe1 protein increased transiently in the presence of ethanol. These results indicated that Swe1 is involved in cell size control in the presence of ethanol, and that a signal produced by ethanol causes a transient up-regulation of Swe1. Further we investigated comprehensively the ethanol-sensitive strains in the complete set of 4847 non-essential gene deletions and identified at least 256 genes that are important for cell growth in the presence of ethanol.

収録刊行物

  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 68(4), 968-972, 2004-04-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10013144275
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    09168451
  • NDL 記事登録ID
    6930576
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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