Cryopreservation of Sakhalin Taimen Hucho perryi Spermatozoa: Effect of Cryoprotectants on Post-thaw Fertility

DOI JASI HANDLE Web Site 21 References Open Access

Bibliographic Information

Other Title
  • イトウ凍結精子における適切な耐凍剤の検討

Search this article

Abstract

To develop the cryopreservation diluent for spermatozoa of the endangered Sakhalin taimen, we examined the effect of the nature of cryoprotectants on post-thaw fertility. Fresh semen was diluted 4 times with each diluent, comprising 90% 300 mM glucose and 10% each cryoprotectant (dimethyl sulfoxide (DMSO), methanol, or glycerol) . The 100μl of diluted semen was dropped on dry ice to freeze and immersed in liquid nitrogen for over 2 h. Four pellets were thawed in 16 ml of a 120 mM NaHCO3 solution at 30°C. Egg batches of 20 g were immediately inseminated with thawed semen (Sperm to egg ratio was 5.0×106: 1) . Semen cryopreserved with methanol showed the highest fertilization rate (82.0±0.9%) among all the cryoprotectants examined. The fertilization rate of semen diluted with DMSO (45.1±2.2%) was significantly higher than that with glycerol (12.7±2.0%) . Semen cryopreserved with methanol yielded almost similar post thaw fertility to that of 50μl of fresh semen (78.4±2.4%) at the sperm to egg ratio of 2.5×106: 1. These results indicate that methanol is the suitable cryoprotectant for Sakhalin taimen spermatozoa.

Journal

  • Aquaculture Science

    Aquaculture Science 52 (2), 171-175, 2004

    Japanese Society for Aquaculture Science

References(21)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top