Expression profiling in hepatocellular carcinoma with intrahepatic metastasis: identification of high-mobility group I(Y) protein as a molecular marker of hepatocellular carcinoma metastasis

• Chuma Makoto

  Pathology Division, National Cancer Center Research Institute Department of Gastroentelology and Hematology, Hokkaido University

• Saeki Norihisa

  Genomics Division, National Cancer Center Research Institute

• Yamamoto Yoshiya

  Pathology Division, National Cancer Center Research Institute Department of Gastroentelology and Hematology, Hokkaido University

• Ohta Tsutomu

  Genomics Division, National Cancer Center Research Institute

• Asaka Masahiro

  Department of Gastroentelology and Hematology, Hokkaido University

• Hirohashi Setsuo

  Pathology Division, National Cancer Center Research Institute

• Sakamoto Michiie

  Pathology Division, National Cancer Center Research Institute Department of Pathology School of Medicine, Keio University

抄録

Hepatocellular carcinoma (HCC) is one of the most common and aggressive human malignancies. Its high mortality rate is mainly a result of intra-hepatic metastases. To investigate the detailed genetic mechanisms in cancer metastasis, we compared the expression profiles of 20 HCCs with intrahepatic metastasis and 10 HCCs without intrahepatic metastasis using an oligonucleotide array. Of the approximately 12600 genes that were analyzed, we identified 34 genes whose expression levels were significantly correlated with intrahepatic metastasis (P < 0.05). Of these genes, we further investigated the expression of high-mobility group I(Y) [HMG-I(Y)] protein. Real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) confirmed that HMG-I(Y) was up-regulated in HCC with intrahepatic metastasis, compared to its level in HCC without intrahepatic metastasis. Further immunohistochemical examination of HMG-I(Y) revealed a significant over-expression in HCC with intrahepatic metastasis, compared with that in HCC without intrahepatic metastasis (P < 0.05). These results indicate that the molecular signatures of HCC with intrahepatic metastasis and of HCC without intrahepatic metastasis are clearly different. HMG-I(Y) expression was associated with intrahepatic metastasis and may be a predictive marker of HCC intrahepatic metastasis.

収録刊行物

• Ｔｈｅ　Ｋｅｉｏ　Ｊｏｕｒｎａｌ　ｏｆ　Ｍｅｄｉｃｉｎｅ

  Ｔｈｅ　Ｋｅｉｏ　Ｊｏｕｒｎａｌ　ｏｆ　Ｍｅｄｉｃｉｎｅ 53 (2), 90-97, 2004

  The Keio Journal of Medicine

キーワード

• HCC
• intrahepatic metastasis
• HMG-I(Y)
• oligonucleotide array

詳細情報

• CRID: 1390282681314590208
• NII論文ID: 10013148354
• NII書誌ID: AA00710216
• DOI: 10.2302/kjm.53.90
• COI: 1:CAS:528:DC%2BD2cXnt1Kqs7w%3D
• ISSN: 18801293
• ISSN: 00229717
• PubMed: 15247513
• Web Site: https://www.jstage.jst.go.jp/article/kjm/53/2/53_2_90/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006013638
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可