Absorption and Metabolism of Quercetin in Caco-2 Cells.

• IKENO Ayumi

  School of Food and Nutritional Sciences, The University of Shizuoka

• HASHIMOTO Kei

  Department of Bioproductive Science, Utsunomiya University

• SHIMIZU Makoto

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• TERAO Junji

  Department of Nutrition, School of Medicine, The University of Tokushima

• KUMAZAWA Shigenori

  School of Food and Nutritional Sciences, The University of Shizuoka

• NAKAYAMA Tsutomu

  School of Food and Nutritional Sciences, The University of Shizuoka

抄録

We investigated the absorption and metabolism of quercetin, a typical flavonoid, in Caco-2 cells, which are obtained from a human colon adenocarcinoma cell line and have been used as a good model for the small intestinal epithelial cells. After incubation of Caco-2 cells with quercetin, cell homogenates were treated with sulfatase type H-5, which releases quercetin from its glucuronides and sulfates. HPLC analysis of the homogenates with an electrochemical detector revealed absorption of quercetin by the cells. The amount of quercetin in the cell homogenates treated with sulfatase type H-5 was much higher than that in the cell homogenates before treatment with the enzyme. These results indicate that quercetin was conjugated easily in Caco-2 cells and suggest that similar conjugation of flavonoids probably occurs in the intestinal epithelial cells.

収録刊行物

• Food Science and Technology Research

  Food Science and Technology Research 5 (1), 90-92, 1999

  公益社団法人 日本食品科学工学会

キーワード

• Caco-2 cell
• conjugated derivatives
• electrochemical detector (ECD)
• flavonoid
• quercetin

詳細情報

• CRID: 1390001204456370176
• NII論文ID: 10013197965
• NII書誌ID: AA11320122
• DOI: 10.3136/fstr.5.90
• ISSN: 18813984
• ISSN: 13446606
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可