Abnormal IL-1 Receptor Antagonist Production in Patients with Polymyositis and Dermatomyositis

この論文にアクセスする

この論文をさがす

著者

抄録

<i>Objective</i> To examine the relationship between serum levels of interleukin-1 receptor antagonist (IL-1Ra) and its gene expression in peripheral blood mononuclear cells (PBMC) from patients with polymyositis and dermatomyositis (PM/DM).<br> <i>Methods</i> IL-1Ra levels in sera from patients and supernatants of unstimulated monocyte cultures were measured by enzyme-linked immunosorbent assay. Expression of IL-1Ra mRNA was analyzed by Northern blotting, and an 86-base pair variable repeat polymorphism in intron 2 of the IL-1Ra gene was determined by polymerase chain reaction.<br> <i>Results</i> Serum IL-1Ra was significantly elevated in 27 patients with active-stage PM/DM when compared with levels in 16 patients with inactive-stage PM/DM and 19 normal controls. Serum concentrations of IL-1Ra were correlated with PM/DM disease activity. IL-1Ra mRNA was detected in freshly isolated PBMC from patients with activestage PM/DM, but not in controls. Moreover, IL-1Ra concentrations were increased significantly in unstimulated monocytes from patients with active-stage PM/DM compared with monocytes from normal controls. However, there were no significant differences in IL-1Ra allele frequencies between patients and normal controls.<br> <i>Conclusion</i> Elevation of both IL-1Ra mRNA and protein in sera of patients with active-stage PM/DM suggest that higher levels of serum IL-1Ra may reflect increased IL-1Ra production in myositis, and that IL-1Ra may regulate IL-1-mediated muscle fiber damage in PM/DM.<br>(Internal Medicine 39: 128-135, 2000)

収録刊行物

  • Internal medicine

    Internal medicine 39(2), 128-135, 2000-02-01

    一般社団法人 日本内科学会

参考文献:  40件中 1-40件 を表示

被引用文献:  1件中 1-1件 を表示

キーワード

各種コード

  • NII論文ID(NAID)
    10013261513
  • NII書誌ID(NCID)
    AA10827774
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09182918
  • NDL 記事登録ID
    5296540
  • NDL 雑誌分類
    ZS21(科学技術--医学--内科学)
  • NDL 請求記号
    Z53-M398
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ