Biphenyl-inducible Promoters in a Polychlorinated Biphenyl-degrading Bacterium, Rhodococcus sp. RHA1

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Five transcriptional promoters of biphenyl-degradation genes in <I>Rhodococcus</I> sp. RHA1 were characterized. We newly identified the <I>etbA4</I> promoter region, which was located adjacent upstream from a ferredoxin reductase gene, <I>etbA4</I> and a dihydrodiol dehydrogenase gene, <I>bphB2</I>. The <I>etbA4</I> promoter activity was determined in RHA1 using a promoter probe vector with a <I>luxAB</I> luciferase reporter gene, and was induced by a variety of aromatic compounds as well as the <I>bphA1</I>, <I>ebdA1</I>, <I>etbA1</I>, and <I>etbD1</I> promoters. All these promoters were induced by aromatic compounds in a closely related heterologous host, <I>R. erythropolis</I> IAM1399 in the presence of RHA1 <I>bphST</I> genes, suggesting that these five promoters are under the control of <I>bphST</I>-coding two-component regulatory system. Sequence comparison of the <I>bphA1</I> promoter with the <I>ebdA1</I> and <I>etbA1</I> promoters, whose transcription starts were determined by primer extension analysis, revealed a consensus sequence centering 42-bp upstream from the transcription start. This consensus was also conserved in the <I>etbA4</I> and <I>etbD1</I> promoters, and deletions of the <I>bphA1</I> promoter affecting the consensus impaired inducible promoter activity. These results suggest that this consensus plays a role in transcription induction and/or the promotion of biphenyl degradation genes in RHA1.

収録刊行物

  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 68(6), 1249-1258, 2004-06-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10013286501
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    6991034
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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